Nuclear reprogramming in cells.

I-12: Nuclear Reprogramming in Bovin Somatic Cell Nuclear Transfer

Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...

I-5: Fifteen Years after Dolly: The Perspectives on Cellular Reprogramming

s:1202:"It is a truly amazing time to developmental biology. During recent decades, three important breakthroughs have been developed: (i) isolation of stem cells from embryo, (ii) animal cloning by nuclear transfer (NT), and (iii) and induced pluripotent stem cells (iPS). Considering these three approaches of "Cellular Reprogramming", it seems that the required elements for cell therapy now ex...

I-10: The Oocyte Express Way to Reprogramming Supports Double Nucleus Transplantation

Studies on cell fusion-mediated nuclear reprogramming have led to the breakthrough of the induced pluripotent stem (iPS) cell technology. While this technology has neared stem cells to applications more than any other method, the mechanistic bases of reprogramming remain largely unsolved. In this context, comparative studies of oocyte and cell fusion-mediated reprogramming hold the greatest pro...

I-8: Somatic Cell Nuclear Reprogramming byMouse Oocytes Endures Beyond ReproductiveDecline

Background: The mammalian oocyte has the unique feature of supporting fertilization and normal development while being able of reprogramming the nuclei of somatic cells towards pluripotency, and occasionally even totipotency. Whilst oocyte quality is known to decay with somatic ageing, it is not a given that different biological functions decay concurrently. In this study, we tested whether ooc...

O-18: Epigenetic Modification of Cloned Embryo Development; State of ART

Background: At the outset of the somatic cell nuclear transfer (SCNT) process, the chromatin structure of the somatic cell which governs its state of differentiation undergoes dramatic changes, called reprogramming, and is compelled back to the embryonic stage. However, the overall epigenetic makeup of the resultant cloned embryos has been acknowledged far different from the fertilized embryos....

Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine